Characterization of L-iPSCs by using gene expression analyses and immunofluorescence staining of H3K27me3.

<p>(A) Quantitative RT-PCR analysis of genes that are typically expressed in mouse naïve (<i>REX1</i> and <i>STELLA</i>) or primed (<i>FGF5</i> and <i>T</i>) pluripotent stem cells. Expression levels of each gene were normalized to GAPDH. The normalized value for ESCs was defined as 1.0. Mean + SD obtained from three independent experiments are shown. (B) Immunofluorescence staining of H3K27me3 as an indicator for X chromosome inactivation. Parent fibroblasts were stained as a positive control. Nuclei surrounded by squares were magnified (left panels). Arrows indicate H3K27me-positive areas, corresponding to inactive X chromosomes. Bar, 50 µm.