Sequence evolution of the intrinsically disordered E7N domain.

<p>(<b>A</b>) Graphical representation of sequence conservation and co-evolution in the E7N domain obtained from the alignment of 207 PV E7 sequences. Sequence stretches that could be aligned with confidence are depicted as sequence logos, and sequence numbering originates in the HPV16 E7 protein. The known functional motifs are shown above the logos as boxes and co-occurring motifs are joined by continuous lines <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047661#pone.0047661-Chemes4" target="_blank">[34]</a>. Pairs of coevolving residues are indicated as filled diamonds, open circles and open triangles, respectively. (<b>B</b>) Highly divergent sequence stretches that could not be aligned with confidence are depicted as histograms of the length of each stretch (upper panel) and as average residue abundances (lower panel). Amino acids are ordered according to decreasing tendency to appear in disordered regions <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0047661#pone.0047661-Brown2" target="_blank">[10]</a>. Linkers 1, 2 and 3 join positions 14 and 21, 28 and 30 and 40 and 51 in the E7 sequence logo, respectively. (<b>C</b>) Distribution of the information content of confidently aligned positions in the E7N domain (average is 2.4±1.0 bits). (<b>D</b>) Distribution of the information content of confidently aligned positions in the E7C domain (average is 2.1±1.2 bits).