Effects of RANKL, TNF-α, and LPS on activation of NF-κB pathway.

<p><b><i>A–C</i></b><i>.</i> Cell lysates of BMMs stimulated with RANKL (150 ng/ml) (<i>A</i>), TNF-α (10 ng/ml) (<i>B</i>), or LPS (1 µg/ml) (<b><i>C</i></b>) for the indicated time periods under the adherent and non-adherent conditions were harvested, then phosphorylation and subsequent degradation of IκB were examined by immunoblot analysis. <b><i>D</i></b><i>.</i> BMMs cultured under the adherent and non-adherent conditions were treated with LPS (1 µg/ml) for 3 hours, after which total RNA was extracted and TNF-α mRNA expression levels were examined by RT-PCR.