NF-κB activation induced by LPS is inhibited by compound 1.

<p>(A) Macrophages were treated or not with compound <b>1</b> (25 μM) and stimulated with LPS (100 ng/mL). After the indicated times nuclear extracts were prepared and the activation of p50 and p65 was evaluated using a TransAM assay. Results represent means ± S.E.M. from stimuli performed in duplicates and are representative of two different experiments. **, <i>P</i> ˂ 0.01, compared with LPS stimulus alone. Macrophages were treated as above and cell extracts were run in SDS-PAGE. IκBα degradation (B) and phosphorylation (C) was detected by immunoblotting. Detection of β-actin was used as loading control. The figures are representative of two different experiments with similar results. (D) Macrophages were stimulated for 30 min with LPS (100 ng/mL) in the presence or absence of compound <b>1</b> (25 μM). Cell extracts were submitted to SDS-PAGE. ERK1/2, JNK, and p38 phosphorylation was detected by immunoblotting. Detection of nonphosphorylated ERK2 was used as loading control. The figures are representative of two different experiments with similar results.