WIN down-regulated MMP-9 mRNA in U937-macrophages.

<p>(a) Quantitative real-time PCR of MMP-9 mRNA. 2 µM and 4 µM WIN decreased MMP-9 mRNA to 66% and 55% respectively. Data are shown as means +/− SD n = 3. **p<0.01 vs. control according to Newman-Keuls Multiple Comparison test following ANOVA. (b) Comparison of MMP-9 mRNA and secreted protein, assessed by real-time PCR (n = 3) and densitometry of Western blot analyses (n = 2). Values of mRNA and secreted MMP-9 Protein are given as percentage of the amounts that were measured in untreated cells at the corresponding time point. Secretion of MMP-9 protein was already decreased to 40% after 1 h, and remained in this range for 24 h. In contrast, no mRNA decrease was observed after 1 h, mRNA level decreased steadily to 55% after 24 h. Data are shown as means +/− SD, *p<0.05, **p<0.01 vs. 1 h according to Newman-Keuls Multiple Comparison test following ANOVA.