Treatment with WIN reduced secretion and activity of MMP-9 in macrophageal differentiated U937 cells.

<p>(a) Western blot analysis of conditioned medium using antibodies against MMP-9 and MMP-12. WIN-treatment resulted in a significant decrease of secreted MMP-9, whereas MMP-12 secretion was not affected. Control cells were treated with vehicle. The figure shows one representative analysis out of three. (b) MMP-9 activity-ELISA of conditioned medium. Upon treatment with 2 µM WIN a reduction of MMP-9 activity was observed, after treatment with 4 µM WIN the reduction was even stronger. Control cells were treated with vehicle. Data are shown as means +/− SD, n = 3. *p<0.05 vs. control, **p<0.01 according to Newman-Keuls Multiple Comparison test following ANOVA. (c) Zymography of conditioned medium. Gelatinolytic activity was strongly decreased by 2 and 4 µM WIN. The figure shows one representative analysis out of three.