MMP-9 expression is downregulated in TC-1 tumors from fat-1 mice.

<p>Total RNA was extracted from TC-1 tumors, followed by reverse transcription. MMP-9 mRNA levels were measured by qRT-PCR. Expression levels of MMP-9 were normalized to β-actin as an internal standard (n = 4 in each group). Asterisks indicate those comparisons (fat-1 vs. wild type (WT) mice) with statistical significance (p<0.05). MMP-9 immunostaining of TC-1 tumors derived from wild type (WT) and fat-1 mice. Bars indicate 200 µm in low-power fields, 50 µm in high-power fields.