Src-catalyzed phosphorylation rates, Src protein content, and Src phosphorylation status in the RWPE1-derived cell lines.

<p>Increasing invasive ability is plotted along the x-axis. (a) Grey bars are phosphorylation rates of peptide 1 by whole cell lysates (normalized by total protein content). White bars are phosphorylation rates by cell lysates due to Src kinase alone after subtracting non-Src background phosphorylation of 1. (b) Total Src content as determined by western blot analysis (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048867#pone.0048867.s005" target="_blank">Fig. S5</a>) (c) Src kinase specific activity as assessed by measured Src activity (Fig. 6A) divided total Src protein content (Fig. 6B). (d) pY416 levels were derived from the band intensities in the western blots (<a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0048867#pone.0048867.s004" target="_blank">Fig. S4</a>), normalized to the corresponding α-tubulin control, and then compared to cell line RWPE1 (RWPE1 as 1). Data are representative of at least three independent experiments and are shown as mean with SEM.