Srk1 is activated in <i>ssp1Δ</i> cells, causing accumulation and phosphorylation of Cdc25.

<p>A. Cell extracts were prepared from <i>ssp1Δ</i>, <i>srk1Δ</i> and <i>ssp1Δ srk1Δ</i> cells and analyzed by Western blot to monitor the levels of Cdc25 with anti-Cdc25 (top) or Cdc2 with anti-PSTAIR antibodies as a loading control (bottom). The graph represents the quantification of the Cdc25 protein level regarding the Cdc2 load control. B. Wild-type (wt), <i>ssp1Δ</i>, <i>cdc25-9A</i> and <i>ssp1Δ cdc25-9A</i> cells were grown in YES media for 3 days at 25°C and 35°C. C. Wild-type (wt), <i>ssp1Δ</i>, <i>cdc25-9A</i> and <i>ssp1Δ cdc25-9A</i> cells were grown at 25°C in YES liquid medium to mid-log phase before being transferred to 35°C for 12 hours and visualized microscopically. Scale bar, 10 μm. D. Length of dividing septated cells of the indicated strains (mean ± SD; n >50 for each value). Stadistic significance compared to wild type performed by t-test analysis of 3 values.