Oenothein B induces CD25 on human T cells.

<p>Human PBMCs (10⁵ cells/well) were treated with the indicated concentrations of oenothein B in X-VIVO medium for 42 hrs. CD25 expression on lymphocytes, which included γδ T cells (CD3+/γδ TCR+), NK cells (CD3−/CD56+), and αβ T cells (CD3+/γδ TCR-), was then measured by flow cytometry. The graph represents pooled data from 5 individuals. Each treatment was analyzed in duplicate and error bars indicate SEM. Significance compared to untreated cells (0 µg/ml) was determined by One-way ANOVA with Bonferroni post-test. *p<0.05, **p<0.01, ***p<0.001.