Oenothein B induces IL-2Rα or CD69 on bovine and human lymphocyte subsets.

<p>(A) Bovine PBMCs (10⁵ cells/well) were treated with the indicated concentrations of oenothein B in X-VIVO medium for 24 hrs, and IL-2Rα expression on γδ T cells and NK cells was measured by multi-color flow cytometry. NK cells were defined as non-γδ T cells that expressed CD335. The graphs represent pooled data from 3 individuals. Each treatment was analyzed in triplicate and error bars indicate SEM. Significance compared to untreated cells (0 µg/ml) was determined by One-way ANOVA with Bonferroni post-test. *p<0.05, **p<0.01, ***p<0.001 (B) Human PBMCs (10⁵ cells/well) were treated with the indicated concentrations of oenothein B in cRPMI medium for 48 hrs. CD69 expression on lymphocytes, which included CD3+ T cells, CD8+ T cells, γδ T cells, and NK cells, was then measured by flow cytometry. The graphs represent pooled data from 5 individuals. Each treatment was analyzed in triplicate and error bars indicate SEM. Significance compared to untreated cells (0 µg/ml) was determined by One-way ANOVA with Bonferroni post-test. *p<0.05, **p<0.01, ***p<0.001.