Calculated EMAST mutation rates for <i>D8S321</i> and <i>D20S82</i> in various MMR backgrounds.

<p>Data from the EGFP-positive population at weeks 4, 5, and 6 time points were used for -4 bp frameshift mutation rate analysis. Single mutation rates were calculated by combining and averaging time-specific mutation rates. Rates are expressed as mutations at microsatellite sequence per cell per generation. Data shown are mean±SEM.</p><p>a, b, c, and d represent significant difference in the mutation rate (<i>P</i><0.05).</p>a<p><i>hMLH1^−/−</i> vs. MMR-proficient (<i>D8S321 P</i> = 0.00012, <i>D20S82 P</i> = 0.01069);</p>b<p><i>hMLH1^−/−</i> vs. <i>hMSH6^−/−</i> (<i>D8S321 P</i> = 0.00012, <i>D20S82 P</i> = 0.01021);</p>c<p><i>hMSH3^−/−</i> vs. MMR-proficient (<i>D8S321 P</i> = 0.00008, <i>D20S82 P</i> = 0.01525);</p>d<p><i>hMSH3^−/−</i> vs. <i>hMSH6^−/−</i> (<i>D8S321 P</i> = 0.0001, <i>D20S82 P</i> = 0.01415). Mutations rates were calculated based on the “method of the mean” developed by Luria and Delbruck <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0050616#pone.0050616-Luria1" target="_blank">[25]</a>.