DNA sequencing results and EMAST mutation frequencies in CRC cells with different MMR backgrounds.

<p>(<b>A</b>) Genomic DNA isolated from EGFP-negative and -positive cells carrying experimental constructs (D8-OF and/or D20-OF) was used to amplify DNA fragments containing EMAST loci to examine if the frameshift mutations had occurred. (<b>B</b>) Mutation frequencies were calculated by dividing the percentage of EGFP positive population in the experimental group (OF) by the percentage of EGFP positive in its negative control (MR-OF) to compute the increase of the EGFP-positive population in folds. There were significantly more EGFP-positive cells in <i>hMLH1</i>- and <i>hMSH3</i>-deficient cells compared to MMR-proficient and/or <i>hMSH6</i>-deficient cells. *: p<0.05.