NFκB-p50 knockdown decreases CD44 expression.

<p>Sum159 cells were transfected with control and NFκB-p50 shRNA constructs and then stained for NFκB-p50 and CD44 expression. Transfection with the control, scrambled DNA shRNA construct (<b>A–E</b>) showed no change in NFκB-p50 expression (<b>B</b>, circle) or CD44 expression (<b>C</b>, circle) when compared to un-transfected cells (arrows). Transfection with the NFκB-p50 shRNA construct (<b>F–J</b>) showed a reduction in NFκB-p50 expression (<b>G</b>, circle) and CD44 expression (<b>H</b>, circle) when compared to un-transfected cells (<b>F–G</b>, arrow).