Effects of inhibition of NFκB translocation into the nucleus and CD44 silencing on NFκB target genes.

<p>(A) TIME cells were transfected with scrambled control siRNA or siRNA against CD44 for 24 h. Then, cells were pretreated for 1 h with 36 µM NFκB SN50 inhibitor or inactive control SN50M peptide, followed by culture into Matrigel for 16 h. RNA was extracted and the mRNA levels of CXCL9, CXCL12, CXCR3 and CXCR4 were determined via real time PCR, as described in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0090921#s2" target="_blank">Materials and Methods</a>. (B) TIME cells expressing CD44 or not, were harvested after 16 h of culture into Matrigel or on plastic dish and RNA was extracted and analysed via real time PCR for the NFκB target genes IL-6, ICAM-1 and VCAM-1. A representative experiment out of three performed in triplicates with similar results is shown ± SD.