Priming of the LCMV-specific CD8 T cell response occurs in the MedLN.

<p>Thy1.1⁺ P14 CD8 T cells were adoptively transferred into naïve Thy1.2⁺ C57BL/6 recipients that were subsequently infected i.p. with LCMV 24 h later. Starting one day after infection, mice were treated i.p. with either 50 µg of FTY720 or vehicle daily. Spleens, ILN, MesLN and MedLN were harvested on day 5 p.i. and analyzed for (A) the frequency of P14 CD8 T cells and (B) the total number of P14 CD8 T cells. *, significantly different (<i>p</i><0.05) as determined by Student t-test. n.s., not significant. Representative data from one of four individual experiments with 3–5 mice per group is shown.