High frequencies of CD62L⁻ LCMV-specific CD8 T cells in the draining LN is not unique to the MedLN.

<p>C57BL/6 mice were infected in the footpad with LCMV. (A) Viral titers in the Ips PopLN (solid upwards triangle), Con PopLN (downwards triangle) and spleen (solid square) were measured via plaque assay at 24, 48 and 72 h p.i.. *, Ips PopLN is significantly greater (<i>p</i><0.05) than all other as determined by <i>ANOVA</i>. (B and D) Naïve Thy1.1⁺ P14 CD8 T cells were adoptively transferred into naïve Thy1.2⁺ recipients and infected via the footpad with LCMV the following day 34–40 p.i., the indicated tissues were harvested and assessed for the frequency (B) GC B cells (as described in <a href="http://www.plospathogens.org/article/info:doi/10.1371/journal.ppat.1003054#ppat-1003054-g005" target="_blank">Figure 5</a>) and (D) CD62L⁺ P14 CD8 T cells. (C) The total number of cells in each LN or spleen was assessed in naïve mice or in mice previously infected (day 34–40) with LCMV via the footpad. (B and C) *, Day 34 Ips PopLN is significantly greater (<i>p</i><0.05) than naïve Ips PopLN determined by <i>ANOVA</i>. (D) *, Ips PopLN is significantly lower (<i>p</i><0.05) than Con PopLN as determined by <i>ANOVA</i>. Combined data from 2–3 individual experiments with four mice per group. Error bars represent the standard error of the mean.