CD62L⁻ effector memory CD8 T cells preferentially traffic to the draining MedLN.

<p>CD62L⁺ and CD62L⁻ memory P14 cells were isolated from LCMV-immune mice. CD62L⁺ cells were CFSE-labeled and mixed with unlabeled CD62L⁻ P14 cells and transferred into either naïve or day 34 LCMV infected mice. 72 h post-transfer, LNs were harvested and transferred cells (CD8⁺Thy1.1⁺) were examined for their ratios in naïve mice or day 34 LCMV-infected mice. (A) Representative histograms from naïve (top) and day 34 LCMV-infected (bottom) mice is shown from one of three experiments with 5 mice total per group. (B) Graphs depicting combined data from all experiments presented in a logarithmic scale. Na?¨ve mice are shown the open bars and day 34-LCMV mice are shown in the closed bars. **, MedLN is significantly lower (<i>p</i><0.01) than the ILN, CLN and MesLN as determined by <i>ANOVA</i>.