Lys158–Lys161 on MOD are responsible for the interaction between maize RIP and P2.

<p><b>A) MOD does not bind to P2 when Lys158–Lys161 are mutated to alanine.</b> SDS-PAGE of the last wash (W) and elution (E) obtained from the pull-down assay of maize RIP and P2. Input indicates the purified proteins loaded to the column. <b>B) Sensorgram showing MOD but not pro-RIP and MOD [K158A-K161A] interact with sensor chip-immobilized P2.</b> 500 nM of maize RIP variants or running buffer were injected to the sensor chip for three minutes for association, followed by a dissociation using running buffer. Response unit (RU) was monitored in a real-time manner.