Interaction between RIPs and P2.

<p><b>A) Interaction of MOD, TCS and StxA with P2 at different ionic strengths.</b> Pull-down assay was conducted on RIPs under various ionic strengths to compare their strength of interaction with P2. Input indicates the same amount of purified RIPs was loaded to the P2-sepharose column and proteins were eluted using buffer with 1M NaCl. <b>B) Interactions between RIPs and C-terminal truncated P2.</b> C-terminal truncated P2 variants were subject to pull down assay with RIPs. The C-terminal amino acid sequences of P2, P2 [ΔC5] and P2 [ΔC10] are: AEEKKDEKKEESEE<b>SDDDMGFGLFD</b>, AEEKKDEKKEESEE<b>SDDDMG</b> and AEEKKDEKKEESEE<b>S</b> respectively. The bold letters refer to the conserved residues in P-proteins.