Summary of chromatin organization in the Elf5 region.

<p>(<b>A</b>) ChIP-seq for H3K4me2 in liver (red), lactating mammary glands (blue) and mammary epithelial cells isolated from 12 week virgin mammary glands (black). Black bars at the top of the Liver, Lactating MG, and Virgin MG panels show the H3K4me2 enriched regions identified by MACS. <b>CpG</b>: genomic locations of CpG sites in the 5′ flanking region, exon1 & part of intron 1 (−1418 to +189); DMR indicates the differentially modified region analyzed. (<b>B</b>) Table: shows average levels of DNA methylation (%) at developmental stages for individual CpG sites in region analyzed by SEQUENOM mass-array. (<b>C</b>) Scatter plot of the changes in DNA methylation levels at different time points during mammary gland development and differentiation at DMR in Elf5 promoter: different developmental time points are depicted with different filled symbols. <i><u>Mammary epithelial cell enriched fractions (MEC</u>)</i>, filled symbols: Virgin (2.5_3V: 2.5–3 week old virgin female, filled circle; 3.5–4V, 3.5–4 week old virgin, filled square; 5.5_6V: 5.5–6 week old female virgin, filled downward pointing triangle; 8_15V; 8–15 week old virgin, filled upward pointing triangle); Pregnancy (7P: 7day pregnant, filled diamond; 16P: 16 days pregnant (whole tissue), half vertically filled circle); Lactation (8L; 8 day lactation (whole tissue), half horizontally filled circle); Inv (>28 day after lactation) 5-pointed star; AMV: Age Matched Virgin (Virgin animal same age as >28 day involuted animal), filled hexagonal. <i><u>Non-MEC cell fraction of the mammary gland</u></i> (Non-MEC, open symbols): 2.5_3: 2.5–3 week old virgin female, open circle; 3.5–4V, 3.5–4 week old virgin, open square; 5.5_6: 5.5–6 week old female virgin, open downward pointing triangle; 8_15; 8–15 Week virgin, open upward pointing triangle; Pregnancy (7P: 7day pregnant, open diamond); Inv (>28 day after lactation, 8 pointed star); AMV: Age Matched Virgin (Virgin animal same age as >28 day involuted animal), open hexagonal); or <i><u>Non-mammary tissue</u></i> (hexagonal with star): Liver.