DNA methylation levels during mammary gland development and differentiation at casein gene promoters.

<p>(<b>A, B</b>) DNA methylation levels (%) for Beta-casein promoter (Csn2) (average of 4 CpG sites in 326 bp), (<b>C,D</b>) Alpha s2b casein promoter (Csn1s2b) (average of 2CpG sites in 300 bp) and (<b>E,F</b>) Kappa casein promoter <i>(Csn3)</i> (average of 5 CpG sites in 588 bp) were determined by bisulfite cloning and sequencing. <i><u>Mammary epithelial Cell enriched fractions (MEC</u>)</i>: Virgin (8_15V; 8–15 week old virgins,); Pregnancy (7P: 7day pregnant females); Inv (>28 day after lactation); AMV: Age Matched Virgin (Virgin animals same age as >28 day involuted animals). <i><u>Whole tissue</u>:</i> pregnancy (16P: 16 days pregnant); Lactation (8L; 8 day lactation). <i><u>Non-mammary gland</u>: none-MEC cell fraction of the mammary gland</i> (8_15; 8–15 Week old virgins); Pregnancy (7P: 7day pregnant females); Inv (>28 day after lactation); AMV: Age Matched Virgin (Virgin animals same age as >28 day involuted animals)); <i>or <u>non-mammary tissue</u></i>: Liver. (<b>A,C,E</b>) Overview of Csn2 (<b>A</b>), Csn1s2b (<b>C</b>) and Csn3 (<b>E</b>) promoter and CpG sites analyzed, Tabular representation of DNA methylation levels (%) at individual CpG sites in analyzed region, and DNA methylation levels (%) heatmap representation (yellow 0%-dark blue 100%);. (<b>B, D, F</b>) Methylation status at CpG sites in Bisulfite-sequence clones for mammary gland or non-mammary gland tissue (yellow 0% methylation, dark blue 100% methylation): (2–6 individual preps (mammary gland MEC (8-15wV 8–15, 7dP, INV, AMV) or Non-Mammary gland epithelial cells (8-15wV_s, 8-15_s, 7dP_s, INV_s, AMV_s)) and 4–6 individual animals (16dp, 8dL, Lvr), 5–10 clones/DNA prep), Csn2 (C), Csn1s2b (E), Csn3 (F).