SseL does not influence degradation and processing of IκBα and p100 in infected primary macrophages.

<p>Macrophages were uninfected (U), exposed to LPS (1 µg/ml) for 2 h or infected with indicated strains of <i>S.</i> Typhimurium. (A) Cells were harvested and lysed at the indicated time-points after bacterial uptake and the levels of IκBα and phospho-IκBα were analysed by immunoblot using anti-IκBα and anti-phospho-IκBα antibodies. The same blots were probed for β-tubulin as a loading control. Immunoblots are representative of three independent experiments. (B) Macrophages were infected with <i>S</i>. Typhimurium strains (wt or Δ<i>sseL</i> mutant) grown to mid-log or stationary phase. Cell lysates were collected at different time-points after bacterial uptake and analysed for IκBα levels by immunoblot using anti-IκBα antibody. The membrane was reprobed with an anti-β-tubulin antibody, as a loading control. Immunoblots are representative of three independent experiments. (C) Macrophages were infected as in (A) and cell lysates were collected at different time-points after bacterial uptake and analysed by immunoblot using anti-p100/p52 antibody. U lane represents uninfected cells. Results are representative of three independent experiments.