EutR enhances S. Typhimurium survival within macrophages.

<p>(<b>A</b>) Intramacrophage survival and replication of <i>S</i>. Typhimurium (AJK61) and the Δ<i>eutR</i> (CJA023) strains after 5 h phagocytosis in RAW macrophages (error bars represent the geometric mean value ± SE of 24 independent experiments). (<b>B</b>) Intramacrophage survival and replication of <i>S</i>. Typhimurium (CJA034), Δ<i>eutR</i> (CJA032), and Δ<i>eutR</i> complemented with <i>eutR</i> (<i>eutR</i>+) (CJA033) strains after 5 h phagocytosis in peritoneal exudate macrophages (PEMs) (error bars represent the geometric mean value ± SE of nine independent experiments). (<b>C</b>) Intramacrophage survival and replication of <i>S</i>. Typhimurium (AJK61), Δ<i>eutR</i> (CJA023) and Δ<i>eutB</i> (CJA028) strains after 5 h phagocytosis in PEMs (error bars represent the geometric mean value ± SE of six independent experiments). (<b>D</b>) <i>In vitro</i> growth curve of S. Typhimurium WT (SL1344), Δ<i>eutR</i> (CJA009), or Δ<i>eutB</i> (CJA020) strains in SPI-2 inducing medium without or with supplementation of 5 mM ethanolamine (EA). Each data point shows the average of three independent experiments. (<b>E</b>) <i>In vitro</i> growth curve of S. Typhimurium WT (SL1344), Δ<i>eutR</i> (CJA009), or Δ<i>eutB</i> (CJA020) strains in tissue culture medium without or with supplementation of 5 mM ethanolamine (EA). Each data point shows the average of three independent experiments. *, <i>P</i> ≤ 0.05; **, <i>P</i> ≤ 0.005; ***, <i>P</i> ≤ 0.0005; <i>P</i> > 0.05 = ns.