GFP reporter expression in T cell subsets of CAG-rtTA3 mice.

<p>Flow cytometry profile of GFP expression in thymic and splenic T cell subsets from a representative CAG-rtTA3 bitransgenic reporter mouse (green) compared with a control mouse (black). Mice were given Dox food for 7 days before analysis. The percentage of GFP+ cells in each population is indicated. (A) Reporter expression during thymocyte differentiation through DN (CD4–CD8–) to DP (CD4+CD8+) to SP (CD4+CD8– and CD4–CD8+) stages. (B) Reporter expression in splenic T cell subsets. Naïve: CD62L+CD44–, Effector: CD62L–CD44+, Effector memory: CD44+CD127+CD62L–, Central memory: CD44+CD127+CD62L+. Gating strategies are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054009#pone.0054009.s005" target="_blank">Figure S5</a>.