Tet-regulated GFP reporter expression in hematopoietic stem cells and early progenitors of tet-transactivator transgenic mice.

<p>Flow cytometry profiles of GFP expression in hematopoietic stem and progenitor cells isolated from the bone marrow of various transgenic mouse strains. Profiles from a representative mouse (n = 2 mice analysed per genotype) carrying the indicated transactivator transgene along with the TRE-GFP-shLuc reporter transgene are shown in green, with wild type controls shown in black. Tet-on bitransgenic reporter mice (CAG-rtTA3, CMV-rtTA, ROSA26-M2rtTA, Vav-rtTA3) were given Dox food for 7 days before analysis, whereas tet-off bitransgenic reporter mice (Eµ-tTA and Vav-tTA) were untreated. The percentage of GFP+ cells in each population is indicated. HSC: Lin–Sca1+Kit+ (LSK) hematopoietic stem cell. CLP: Lin–Kit^IntSca1+CD127+ common lymphoid progenitor. CMP: Lin–Sca1–Kit+CD34+FcγRII/III– common myeloid progenitor. GMP: Lin–Sca1–Kit+CD34+FcγRII/III+ granulocyte/macrophage progenitor. MEP: Lin–Sca1–Kit+CD34–FcγRII/III– megakaryocyte/erythroid progenitor. MkP: Lin–Sca1–Kit+CD41+CD150+ megakaryocyte progenitor. Gating strategies are shown in <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0054009#pone.0054009.s002" target="_blank">Figure S2</a>.