Flow cytometric characterization of ChAT-GFP+ T-cells.
- Valerie T. Ramirez (474026)
- Dayn R. Godinez (6623513)
- Ingrid Brust-Mascher (52631)
- Eric B. Nonnecke (4759920)
- Patricia A. Castillo (128473)
- Mariana Barboza Gardner (6580943)
- Diane Tu (6580946)
- Jessica A. Sladek (6580949)
- Elaine N. Miller (6623516)
- Carlito B. Lebrilla (223528)
- Charles L. Bevins (128491)
- Melanie G. Gareau (403140)
- Colin Reardon (4285078)
Publication date
April 2019
DOI Publisher
Public Library of Science (PLoS)Abstract
(A) Lamina propria lymphocytes were isolated from ChAT-GFP mice 10 days after C. rodentium infection, or (LB gavaged) controls. Representative gating strategy depicts analysis of (lymphocytes, single, cells, live CD3+). CD4+ ChAT-GFP+ or CD4+ ChAT-GFP- cells expressing IFNγ, IL-17A, or IL-22 in each of these populations (light grey: non-stimulated control, green; ChAT-GFP+ cells stimulated with PMA/ionomycin, dark grey: ChAT-GFP- cells stimulated with PMA/ionomycin). The amount of each cytokine was quantified by MFI (B), and the frequency of these cells per live CD3+ cell (C). * P < 0.05 ANOVA, with n = 4–6 mice per group.</p
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