Therapy with R-LM249 of systemic metastases induced by the i.v. injection of human breast carcinoma cell line MDA-MB-453.

<p>R-LM249 was administered i.p. at 10⁸ pfu dose (4 weekly injections) to groups of 9–10 Rag2^−/−;Il2rg^−/− mice. (<b>A, C, E</b>) incidence of macroscopic metastases in the indicated organs, as determined at necropsy; (<b>B, D, F</b>) total metastatic burden (mean + SEM) in organs as quantified by human centromeric DNA qPCR. Statistical analysis of differences: panel <b>A</b>, p<0.0001 at Fisher's exact test; panel <b>B</b>, p = 0.0004 at both Student's <i>t</i> and non-parametric Wilcoxon rank sum tests; panel <b>C</b>, p = 0.07 at Fisher's exact test; panel <b>D</b>, p = 0.056 at Student's <i>t</i> test and p = 0.03 at non-parametric Wilcoxon rank sum test.