Therapy with R-LM249 of Rag2^−/−;Il2rg^−/− mice bearing i.p. human SK-OV-3 ovarian carcinoma.

<p>(<b>A–C</b>) incidence of peritoneal carcinomatosis, weight of metastatic lesions (mean + SEM) and incidence of ascites fluid in groups of 5–7 mice. Statistically significant differences: panel <b>B</b>, p = 0.007 at Student's <i>t</i> test; panel <b>C</b>, p = 0.027 at Fisher's exact test. (<b>D</b>) control mouse, treated with vehicle (PBS) alone, showing multiple i.p. masses (green arrows); (<b>E</b>) tumor-free mouse treated with R-LM249; (<b>F</b>) distribution of R-LM249 in a mouse bearing multiple i.p. tumors shows that the virus selectively populates and replicates in the HER-2+ masses (green fluorescence: virally-encoded EGFP; yellow-brown florescence: autofluorescence of mouse fur and visceral organs).