Agarose gel electrophoresis for nested PCR analysis of HCMV-DNA.

<p>Samples were considered positive when a band of 293bp was detected. (<b>A</b>) Representatives of non-IBC carcinoma tissue samples: lanes 1–7 display positive-HCMV; lanes 8–10 represent negative samples. (<b>B</b>) Representatives of IBC carcinoma tissue samples: lanes from 1–4, 6–7, 9–10 display positive HCMV; lanes 5 and 8 represent negative samples. Lanes +C represent HCMV positive control; lanes -C represent HCMV negative control. (<b>C</b>) Nucleotide sequence alignment from the purified nested PCR products. *represents nucleotide identity.