<p>HCV genes core, F, NS3, NS4, NS5a or NS5b are not amplified. First panel shows the DNA ladder, followed by agarose gel electrophoresis of PCR products obtained with HCV core, F, NS3, NS4, NS5a and NS5b specific primers. HEK lysate supernatant and rAd-HCV vectors were used as negative and positive controls. Data are representative of 2–3 repeated experiments.</p
Introduction Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) R...
<p>To determine whether HCV could infect DHCs, we infected cells with either HCVcc or serum from CHC...
<p>Primers used in RT-PCR, and PCR reactions of the 5′UTR and NS5B region of HCV and the nested ampl...
<p>(a) Electrophoretic profile of the product of RT-PCR amplification of the NS3 region of HCV. (b) ...
<p>PCR products were separated on 1.5% agarose gel. Three different primer sets were used to amplify...
<p>Samples were considered positive when a band of 293bp was detected. (<b>A</b>) Representatives of...
<p>(A) HCV replicon cells were transfected with empty vector (lane 2) or the plasmid expressing cdN ...
HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genom...
HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genom...
<p>(A) HCV RNA positive strand identification using 2 μg of total RNA extracted from PBMC treated <i...
<p>A SYBR-green assay and plasmid standard was used for the CAV and CPV samples, whilst the HCV and ...
OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients...
<p>“L” represents the 100-bp DNA ladder ranging from 100–1000 bp. Lanes 1, 2, 3, 4, and 5 contain DN...
<p>A. The genome replication inhibitors (Inhs 6–13) were confirmed by comparing potency (EC<sub>50</...
<p>(<b>a</b>) Schematic outline of the viral vector constructs and location of real-time PCR primers...
Introduction Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) R...
<p>To determine whether HCV could infect DHCs, we infected cells with either HCVcc or serum from CHC...
<p>Primers used in RT-PCR, and PCR reactions of the 5′UTR and NS5B region of HCV and the nested ampl...
<p>(a) Electrophoretic profile of the product of RT-PCR amplification of the NS3 region of HCV. (b) ...
<p>PCR products were separated on 1.5% agarose gel. Three different primer sets were used to amplify...
<p>Samples were considered positive when a band of 293bp was detected. (<b>A</b>) Representatives of...
<p>(A) HCV replicon cells were transfected with empty vector (lane 2) or the plasmid expressing cdN ...
HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genom...
HCV replicates in liver via an intermediate negative strand RNA. To study the relevance of HCV genom...
<p>(A) HCV RNA positive strand identification using 2 μg of total RNA extracted from PBMC treated <i...
<p>A SYBR-green assay and plasmid standard was used for the CAV and CPV samples, whilst the HCV and ...
OBJECTIVE: To study the variation of HCV 5'-NCR and core gene in serial sera collected from patients...
<p>“L” represents the 100-bp DNA ladder ranging from 100–1000 bp. Lanes 1, 2, 3, 4, and 5 contain DN...
<p>A. The genome replication inhibitors (Inhs 6–13) were confirmed by comparing potency (EC<sub>50</...
<p>(<b>a</b>) Schematic outline of the viral vector constructs and location of real-time PCR primers...
Introduction Molecular biology procedures to detect, genotype and quantify hepatitis C virus (HCV) R...
<p>To determine whether HCV could infect DHCs, we infected cells with either HCVcc or serum from CHC...
<p>Primers used in RT-PCR, and PCR reactions of the 5′UTR and NS5B region of HCV and the nested ampl...