<p>(<b>A</b>) Far-UV CD spectra of PhaF in the absence (solid line) and the presence (dashed line) of 4.1 M urea. (<b>B)</b>. CD-monitored urea titration of 3.8 µM PhaF (closed circles), 11.4 µM PhaF (open triangles) and C-PhaF (open circles). The solid lines represents the fitting to a two-state equilibrium denaturation transition (see text).</p
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear depen...
<p>(A) Urea concentrations for the labels 1–9 were 0, 1, 2, 3, 4, 5, 6, 7 and 8 M, respectively. (B)...
<p>(<b>A</b>) Far-UV CD spectra of PhaF at different temperatures. Recovered spectra were registered...
<p>(<b>A</b>) Far-UV CD spectra of PhaF registered at different pH's. (<b>B</b>) pH titration of Pha...
<p>Unfolding transition curves monitored by (A) ellipticity at 222 nm and (B) the tryptophan fluores...
<p>Thermal stabilities were studied monitoring the changes by (<b>a</b>) far-UV CD at 222 nm and by ...
<p>Unfolded fraction (f<sub>Unf</sub>) of wild type PpAzoR (circles) and 1B6 (squares), 2A1 (diamond...
The black line corresponds to the apo-C14A/M69F double mutant and the red line corresponds to the ap...
<p>Unfolding was induced by urea (A, C) or GdmCl (B, D). Protein variants were incubated with denatu...
<p>Melting temperatures and thermodynamic parameters for urea-induced unfolding equilibrium of PGK1 ...
<p>(A) Representative Trp fluorescence wavelength scans of hVDAC-2 WT (left) and C0 (right) refolded...
<p>(A) Urea induced unfolding transition curves of BL at pH 7.4 (-▪-) and pH 2.0 (–▴–) monitored thr...
<p>Far- UV CD spectrum of Full length P2 (filled circles ·), Deletion construct of P2 (open circles ...
Equilibrium unfolding of barstar with guanidine hydrochloride (GdnHCl) and urea as denaturants as we...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear depen...
<p>(A) Urea concentrations for the labels 1–9 were 0, 1, 2, 3, 4, 5, 6, 7 and 8 M, respectively. (B)...
<p>(<b>A</b>) Far-UV CD spectra of PhaF at different temperatures. Recovered spectra were registered...
<p>(<b>A</b>) Far-UV CD spectra of PhaF registered at different pH's. (<b>B</b>) pH titration of Pha...
<p>Unfolding transition curves monitored by (A) ellipticity at 222 nm and (B) the tryptophan fluores...
<p>Thermal stabilities were studied monitoring the changes by (<b>a</b>) far-UV CD at 222 nm and by ...
<p>Unfolded fraction (f<sub>Unf</sub>) of wild type PpAzoR (circles) and 1B6 (squares), 2A1 (diamond...
The black line corresponds to the apo-C14A/M69F double mutant and the red line corresponds to the ap...
<p>Unfolding was induced by urea (A, C) or GdmCl (B, D). Protein variants were incubated with denatu...
<p>Melting temperatures and thermodynamic parameters for urea-induced unfolding equilibrium of PGK1 ...
<p>(A) Representative Trp fluorescence wavelength scans of hVDAC-2 WT (left) and C0 (right) refolded...
<p>(A) Urea induced unfolding transition curves of BL at pH 7.4 (-▪-) and pH 2.0 (–▴–) monitored thr...
<p>Far- UV CD spectrum of Full length P2 (filled circles ·), Deletion construct of P2 (open circles ...
Equilibrium unfolding of barstar with guanidine hydrochloride (GdnHCl) and urea as denaturants as we...
<p>(A) The folded protein in the absence of urea (solid line) compared with the unfolded protein in ...
<p>The urea-induced unfolding was analyzed in 20 mM po4 buffer pH7.5, assuming ΔG has a linear depen...
<p>(A) Urea concentrations for the labels 1–9 were 0, 1, 2, 3, 4, 5, 6, 7 and 8 M, respectively. (B)...