Agarose gel analysis of hsp70 PCR-RFLP from different parasite isolates.

<p>The PCR products of 1420 bp were digested with the restriction enzyme HaeIII. Digested products were separated on 3% Small Fragment Agarose (Sigma,India). Lane M is a 100 bp Ladder (New England Biolabs, UK). Lanes 2- confirmed <i>L. donovani</i> (BHU-744), Lane 3: confirmed <i>Leptomonas</i> (BHU-154). Lane 4 is a BHU-1095 showing <i>L. donovani</i> signature. Lanes 5 and 6: BHU 39 and BHU 2001 respectively.