Agarose gel electrophoresis of : a) the PCR products from the Hexokinase (HK) genes of leishmanial reference strains after their double digestion with the endonucleases <i>MboI</i> and <i>HaeIII</i>, nd = not done; b) of PCR products from the HK genes of the leishmanial references and local Israeli and Palestinian strains, and Palestinian clinical samples after their double digestion with the endonucleases <i>MboI</i> and <i>HaeIII</i>.

<p>Uncut PCR product from <i>L. tropica</i> MHOM/PS/18JnMF4 ( = LRC-L891) was used as a control and molecular sizes were compared with a 50 bp molecular weight ladder (MW), nd = not done; c) of the PCR products from the Phosphoglucomutase (PGM) genes of the leishmanial reference strain <i>L. tropica</i> LRC-L863 and local Palestinian strains after single digestion with the endonuclease <i>MboI</i>. Only the heavier differentiating band is shown. Uncut PCR product from <i>L. tropica</i> MHOM/PS/02/79JnF20 ( = LRC-L885) was used as a control and molecular sizes were compared with a 50 bp molecular weight ladder (MW).