PCR product sizes of MS-H1 and MS-H2 agarose-MAMAs in agarose gel.

<p>Electrophoresis was performed in 3% agarose gel (MetaPhor Agarose, Lonza Group Ltd., Basel, Switzerland) and a 20-bp DNA ladder (O'RangeRuler 20 bp, Thermo Fisher Scientific Inc.) was used as molecular weight marker (m). In the MS-H1 assay gBlock-B and a wild-type strain yielded 71 bp fragments, while gBlock-A and the temperature sensitive MS-H vaccine strain produced 91 bp fragments. In the MS-H2 assay gBlock-B (non-temperature sensitive MS-H re-isolate homologue) yielded 77 bp fragment, while gBlock-A, the temperature sensitive MS-H vaccine strain and wild-type strains give 96 bp fragments.