<p>(A) Agarose gel (2.0%) electrophoresis indicates amplification of a single PCR product of the expected size for 13 genes. (B) Melting curves of 13 genes show single peaks. M represents 100 bp DNA marker.</p
<p>GeneRuler™1 kb Plus DNA markers (M, in bp) and typical DNA samples isolated by SDS (1), CTAB (2),...
(A) Agarose gel electrophoresis images of PCR mixtures at various annealing temperatures (30–55°C) u...
<p>Amplified fragments shown on 1% agarose gel electrophoresis with ethidium bromide staining. An id...
<p>(a) Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expec...
<p>(A) Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expec...
<p>(a) Melting curves of ten candidate reference genes and one target gene showing single peaks. (b)...
<p>Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expected ...
a). The agarose gel (2.0%) electrophoresis displaying a single PCR product with expected sizes for s...
<p>A: Melt curves for candidate reference genes. B: Agarose gel (2%) showing amplification of a sing...
<p>(A) The first polymerase chain reaction with 3% ramp speed was amplified by specific sequences, a...
<p>A) Melting curve of the 11 reference genes showing a single pick (each including three technical ...
<p>Three replicates of the PCR amplicons with each primer set were loaded; M indicates a100 bp DNA s...
<p>All of the amplicons showed a single peak in melting curve analysis. After qPCR cycles, a melting...
<p>(A) (<i>Lane 1</i>) 50 bp DNA ladder (Fermentas, USA) for size determinations; (<i>Lane 2</i>) Sb...
<p><b>A</b>) Melting curve of the 11 reference genes showing a single pick (each including three tec...
<p>GeneRuler™1 kb Plus DNA markers (M, in bp) and typical DNA samples isolated by SDS (1), CTAB (2),...
(A) Agarose gel electrophoresis images of PCR mixtures at various annealing temperatures (30–55°C) u...
<p>Amplified fragments shown on 1% agarose gel electrophoresis with ethidium bromide staining. An id...
<p>(a) Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expec...
<p>(A) Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expec...
<p>(a) Melting curves of ten candidate reference genes and one target gene showing single peaks. (b)...
<p>Agarose gel (2%) electrophoresis showing amplification of a specific PCR product of the expected ...
a). The agarose gel (2.0%) electrophoresis displaying a single PCR product with expected sizes for s...
<p>A: Melt curves for candidate reference genes. B: Agarose gel (2%) showing amplification of a sing...
<p>(A) The first polymerase chain reaction with 3% ramp speed was amplified by specific sequences, a...
<p>A) Melting curve of the 11 reference genes showing a single pick (each including three technical ...
<p>Three replicates of the PCR amplicons with each primer set were loaded; M indicates a100 bp DNA s...
<p>All of the amplicons showed a single peak in melting curve analysis. After qPCR cycles, a melting...
<p>(A) (<i>Lane 1</i>) 50 bp DNA ladder (Fermentas, USA) for size determinations; (<i>Lane 2</i>) Sb...
<p><b>A</b>) Melting curve of the 11 reference genes showing a single pick (each including three tec...
<p>GeneRuler™1 kb Plus DNA markers (M, in bp) and typical DNA samples isolated by SDS (1), CTAB (2),...
(A) Agarose gel electrophoresis images of PCR mixtures at various annealing temperatures (30–55°C) u...
<p>Amplified fragments shown on 1% agarose gel electrophoresis with ethidium bromide staining. An id...
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