Specificity of finger millet reference gene primer pairs for RT-qPCR amplification.
- Palakolanu Sudhakar Reddy (530583)
- Mahamaya G. Dhaware (5856095)
- Dumbala Srinivas Reddy (475340)
- Bommineni Pradeep Reddy (5856098)
- Kummari Divya (5856101)
- Kiran K. Sharma (475343)
- Pooja Bhatnagar-Mathur (475341)
Publication date
October 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
a). The agarose gel (2.0%) electrophoresis displaying a single PCR product with expected sizes for sixteen-finger millet reference genes. M represents the DNA size marker (left side 100 bp DNA marker and right side 50 bp DNA ladder). b). Melt curves of sixteen finger millet reference genes displaying a single and sharp peaks generated from the amplicons.</p
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