The experimentally measured affinity and affinity change are highly correlated with the predicted values.

<p>(<b>A</b>) The correlation between the TBP/TATA affinity as predicted <i>in silico</i> and measured <i>in vitro.</i> The coefficient of linear correlation, r, is 0.822 at a significance level of α <10⁻⁷. (<b>B</b>) The correlation between SNP-caused TBP/TATA affinity change (δ) as predicted <i>in silico</i> and measured <i>in vitro.</i> The coefficient of linear correlation, r, is 0.785 at a significance level of α<10⁻³. 95% confidence intervals for linear regression built using the package STATISTICA.