Experimental measurements of the equilibrium <i>K</i>_D for hTBP/TATA complexes.

<p>The use of an electrophoretic mobility shift assay for measuring hTBP/TATA affinity: (<b>A</b>) a TATA-containing ODN in healthy people (the −21C allele); (<b>B</b>) an ODN with the TATA box containing the SNP associated with myocardial infarction and thrombophlebitis (the −21t allele); (<b>C</b>) TBP/TATA binding isotherms inferred from electrophoregrams using OriginPro 8.