<b>Mobilization of T cells is strain and TLR2-dependent.</b>

<p> F1 (SJLxB6^wt), F1 (SJLxB6^tlr−) (<b>A</b>, <b>C</b>) or SJL (B, <b>D</b>) mice were immunized with p139 in IFA in the presence of the indicated amounts of killed <i>M tb</i> (<b>A</b>), or of PPD (<b>B</b>, <b>C</b>) or of a 1∶1 w/w mixture of PAM2-(CSK)3 and PAM3-(CSK)3 (<b>D</b>). The number of mice for each group is indicated in the figure. Fourteen days later mice were sacrificed and the presence of T cells carrying the public TCR-beta chain in LN (closed symbols) and spleen (open symbols) was measured by immunoscope. Data are reported as RSI for the peak corresponding to the public BV10 TCR-beta chain for each individual mouse. Dashed lines indicate the cut off value for positivity (established as described in Results). <b>E</b>: 4 F1 (SJLxB6^wt, circles) mice and 5 F1 (SJLxB6^tlr2−, triangles) mice were immunized with regular CFA in PBS. Two weeks later cells from draining LN (full symbols) and spleen (open symbols) were recovered, labeled with CFSE and cultured in the presence or absence (background) of PPD. After 3 days, the number of CD3⁺ cells that had specifically divided in response to PPD was determined as % of CFSE^low CD3⁺ cells over total CD3⁺ cells in the PPD stimulated sample minus % of CFSE^low CD3⁺ cells over total CD3⁺ cells in background sample.