The effects of TLR2 and TLR7 ligands on anti-CD3- activated γδ T cells isolated from MyD88^−/− and TLR7^−/− mice.

<p>Splenic γδ T cells of MyD88^−/− (<i>A</i>–<i>D</i>) or TLR7^−/− mice (<i>E</i>–<i>H</i>) were cultured with anti-CD3 with or without TLR agonists. Cells were harvested at 48 h post-stimulation and analyzed for CD69 expression (<i>A</i>) & <i>(E)</i> and IFN-γ (<i>B</i>) & (<i>F</i>), IL-2 (<i>C</i>) & (<i>G</i>) and TNF-α (<i>D</i>) & (<i>H</i>) production in culture supernatant. Data are presented as means ± SEM, <i>n</i> = 3–4. ** <i>P</i><0.01 or * <i>P</i><0.05 compared to anti-CD3- treated cells. Results presented are one representative of three similar experiments.