<p>Values are given as the percentage of activity (PC<sub>2</sub> synthesized µmol min<sup>−1</sup> mg<sup>−1</sup> enzyme) of respective enzyme in the absence of added metal in the presence of DTPA (6.3 mM). Mean value of three separate assays (± SE) are given (N = 3). The incubation mixtures contained 200 mM Tris-HCl (pH 8), 10 mM β-Me, 10 mM GSH, 3 µg of enzyme and the metal ion.</p>*<p>, p<0.05 by Student's t test.</p
Batch microcalorimetry has been employed to obtain a calibration curve for the enzymatic activity of...
<p>Reaction mixtures (100 µl) containing 11 µg enzymes, 1 µM iAs<sup>3+</sup>, 7 mM GSH in PBS (25 m...
<p>(a) Each of the indicated compounds (2 mM) was incubated with metal-removed TON_0340 (1 μM) at 37...
<p>The incubation mixture contained 200 mM Tris-HCl (pH 8), 10 mM β-Me, 10 mM GSH, 0.1 mM CdCl<sub>2...
<p>(A),(B),(C) and (D) represent the effect of Zn<sup>2+</sup>, Ca<sup>2+</sup>, Fe<sup>3+</sup> and...
<p>The enzyme was incubated with each metal ion or chemical agent at 45°C for 5 min, and residual ac...
<p>Enzyme activities were determined in the presence of 3 mM UDP using the discontinuous apyrase act...
<p>(a) In order to test the effect of pH on CaN activity, CaN assay was performed at different pH. O...
A, The specificity of the enzyme towards different phosphate (P)—containing compounds assayed at 1mM...
<p>Phosphatase activity was measured using <i>p</i>-NPP. A) Magnesium dose-dependent increases of ac...
<p>Rates of substrate hydrolysis were measured in standard reaction mixtures except that Mn<sup>2+</...
<p>The maximum value was considered to be 100%. The values presented correspond to the mean values o...
<p>(A) Effect of different metal ions on the phosphodiesterase activity of PdeM. The metals as well ...
<p><b>A.</b> 10 nM of recombinant protein were incubated with 10 nM of Poly(A) at 37°C for 5 and 60 ...
<p>Reaction mixtures (100 μl) containing 11 μg enzymes, 1 μM MMA<sup>3+</sup>, 1 mM SAM, and 7 mM GS...
Batch microcalorimetry has been employed to obtain a calibration curve for the enzymatic activity of...
<p>Reaction mixtures (100 µl) containing 11 µg enzymes, 1 µM iAs<sup>3+</sup>, 7 mM GSH in PBS (25 m...
<p>(a) Each of the indicated compounds (2 mM) was incubated with metal-removed TON_0340 (1 μM) at 37...
<p>The incubation mixture contained 200 mM Tris-HCl (pH 8), 10 mM β-Me, 10 mM GSH, 0.1 mM CdCl<sub>2...
<p>(A),(B),(C) and (D) represent the effect of Zn<sup>2+</sup>, Ca<sup>2+</sup>, Fe<sup>3+</sup> and...
<p>The enzyme was incubated with each metal ion or chemical agent at 45°C for 5 min, and residual ac...
<p>Enzyme activities were determined in the presence of 3 mM UDP using the discontinuous apyrase act...
<p>(a) In order to test the effect of pH on CaN activity, CaN assay was performed at different pH. O...
A, The specificity of the enzyme towards different phosphate (P)—containing compounds assayed at 1mM...
<p>Phosphatase activity was measured using <i>p</i>-NPP. A) Magnesium dose-dependent increases of ac...
<p>Rates of substrate hydrolysis were measured in standard reaction mixtures except that Mn<sup>2+</...
<p>The maximum value was considered to be 100%. The values presented correspond to the mean values o...
<p>(A) Effect of different metal ions on the phosphodiesterase activity of PdeM. The metals as well ...
<p><b>A.</b> 10 nM of recombinant protein were incubated with 10 nM of Poly(A) at 37°C for 5 and 60 ...
<p>Reaction mixtures (100 μl) containing 11 μg enzymes, 1 μM MMA<sup>3+</sup>, 1 mM SAM, and 7 mM GS...
Batch microcalorimetry has been employed to obtain a calibration curve for the enzymatic activity of...
<p>Reaction mixtures (100 µl) containing 11 µg enzymes, 1 µM iAs<sup>3+</sup>, 7 mM GSH in PBS (25 m...
<p>(a) Each of the indicated compounds (2 mM) was incubated with metal-removed TON_0340 (1 μM) at 37...