HDAC inhibition by TSA decreases SREBP2 proteolytic cleavage.

<p>A) Western blot analysis of SREBP2 protein levels in SH-SY5Y cells after treatment with 250 nM TSA for the indicated time points. The levels of GAPDH are shown as a loading control. B) Quantification of the relative levels of SREBP2 after TSA treatment. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0053394#s3" target="_blank">Results</a> are expressed as fold change relative to vehicle-treated cells and represent means ± SEM from at least three individual experiments. C) NT2-N <i>post-mitotic</i> neurons were treated with 250 nM TSA or vehicle for 16 h. mRNA transcript levels of HMGCR were analyzed by real-time RT-PCR. Values were normalized to the internal standard β-actin and expressed as fold change relative to vehicle-treated cells. Values represent means ± SEM from at least three individual experiments (* <i>p</i><0.05, ** <i>p</i><0.01, *** <i>p</i><0.001).