TSA treatment increased the expression of EMT-related factors with concomitant hyper-acetylation of histone 3 at different time points.

<p>(A) Total RNA was isolated from LNCaP cells treated with 200 nM and 400 nM TSA for 8, 16 and 24 h. The relative mRNA expression of ZEB1, Slug, vimentin and N-cadherin was increased following TSA treatment compared with DMSO control (the value of control was designed as 1, *, p<0.05; **, p<0.01). (B) LNCaP cells were treated with 400 nM TSA for 8 h to 72 h. Western blot analysis showing that TSA treatment promoted acetylation of histone3 (Ac-H3) at 8 h and 16 h treatment. The expression of mesenchymal markers such as Fibronectin (Fibron) and vimentin was elevated after 24 h treatment with TSA. (C) PC3 cells were treated with 400 nM TSA for 4 h to 32 h. Hyper-acetylation of histone 3 was seen at 4 h to 32 h treatment with concomitant increased expression of ZEB1 in each time point of treatment. Enhanced expression of vimentin was observed after 16 h of treatment with TSA.