Representative photographs and microphotographs of the liver.

<p>A clear change in color and general texture was easily appreciate when comparing the liver of animals treated with control diet (<b>A,B</b>) or with the CDE one (<b>C,D</b>). The liver of animals in the CDE Group presents a large number of tumors (<b>C</b>). Tumors in Group CDE+MVC were less numerous and much smaller than those in the previous Group (<b>D</b>). Scale bar for A–D = 1 cm. Histological images were stained with hematoxylin-eosin (<b>E–H</b>), with the fluorescent TUNEL technique (<b>I–L</b>), anti Ki67 (<b>M–P</b>), or with anti-CCR5 antibody (Q–T). The first 2 Groups; Control and MVC (<b>E,F</b>) displayed a normal liver morphology. The liver of the CDE Group had numerous atypic cells and frank tumors (<b>G</b>). Animals treated with MVC had intermediate characteristics (<b>H</b>). Scale bar for E–H = 100 µm. The TUNEL technique detected few apoptotic cells in the liver of animals belonging to control Groups (<b>I,J</b>) but the number increased in animals treated with CDE (<b>K</b>) and was reduced by treatment with MVC (<b>L</b>). Scale bar for I–L = 200 µm. The proliferation marker Ki67 detected few cells in control animals (<b>M,N</b>) and great numbers of positive cells in the CDE Group (<b>O</b>). The number of proliferating cells was intermediate in the CDE+MVC Group (<b>P</b>). CCR5 expression was not detected in control Groups (<b>Q,R</b>) but was found in macrophages (arrowheads), HSC (arrows), and other cell types in the CDE (<b>S</b>) and CDE+MVC (<b>T</b>) Groups. Scale bar for M–T = 100 µm.