Representative images of liver sections of different experiments.

<p>(<b>A–E</b>): Immunohistochemical detection of COX-2, iNOS, NF-κB, MMP-9 and α-SMA (x100). Control gp: minimal expression; CCl₄ gp: extensive expression; BCA+CCl₄ gp: less than CCl₄ gp; BCA gp: minimal expression. Image analysis was performed by examining 6 fields/slide. (<b>F</b>): Masson trichrome stain (x40). Control gp: shows absence of collagen fibers (stained blue) between hepatic lobules; CCl₄ gp: extensive fibers deposition with pseudolobules formation (bridging fibrosis); BCA+CCl₄ gp: less fibers than CCl₄ gp; BCA gp: absence of fibers. The bar chart represents levels of hydroxyproline expressed as µg/gm of wet tissue measured by Reddy’s method. <i>a</i>: significantly different from control gp. <i>b</i>: significantly different from CCl₄ gp.