Expression of autophagy-related protein in cardiomyocytes varies with that of the <i>beclin-1</i> gene.

<p>The relative expression of autophagy-related protein in cardiomyocytes was analyzed by Western blotting. GAPDH was used as an internal control. Data are presented as means ± SEM. (<b>A</b>) Evaluation of the influence of 3-MA on the expression of LC3II/LC3I and beclin-1 proteins. Con: control group, Ang II: treated with 1 µmol/L Angiotensin II, and Ang II+3-MA: treated with 1 µmol/L Angiotensin II and 10 mmol/L 3-MA. *<i>P</i><0.05 compared with Con group; # <i>P</i><0.05 compared with Ang II group. (<b>B</b>) Evaluation of the influence of <i>beclin-1</i> specific siRNA on the expression of autophagy-related proteins. NC: treated with lentivirus containing negative control of <i>beclin-1</i>-specific siRNA, Ang II+ NC: treated with lentivirus containing negative control of <i>beclin-1</i>-specific siRNA and 1 µmol/L Angiotensin II, and Ang II+siRNA: treated with lentivirus containing <i>beclin-1</i>-specific siRNA and 1 µmol/L Angiotensin II. *<i>P</i><0.05 compared with NC group; # <i>P</i><0.05 compared with Ang II+NC group. (<b>C</b>) Evaluation of the influence of pRc/CMV2-beclin-1 vector on LC3II/LC3I and beclin-1 proteins. pRc/CMV2: transfected with pRc/CMV2 vector, and pRc/CMV2-beclin-1: transfected with pRc/CMV2-beclin-1. *<i>P</i><0.05 compared with pRc/CMV2 group.