Effects of ATG9A and miR-34a expression on Ang II-induced autophagic activity in cardiomyocytes.

<p>(<b>A</b>) Flow cytometry. The autophagic vacuoles were detected by using flow cytometry with an anti-autophagy marker protein (LC3) in the cardiomyocytes. The graph summarizes the data of flow cytometry. *P<0.05 compared with Control group. (<b>B</b>) Flow cytometry. The graph summarizes data from the flow cytometry. *P<0.05 compared with NC group, ^#P<0.05 compared to Ang II + NC. (<b>C</b>) Flow cytometry. The graph summarizes data from the flow cytometry. *P<0.05 compared with NC group, ^#P<0.05 compared to Ang II + NC. (<b>D</b>) TEM. The autophagic vacuoles were detected and calculated by transmission electron microscopy (as indicated by the arrows). Magnification of images: ×13500. The graph is the quantitative data from the transmission electron microscopy. Data are presented as means ± SEM *P<0.05 compared with Control group; ^#P<0.05 compared to Ang II + NC. (<b>E</b>) TEM. Magnification of images: ×13500 and ×46000, respectively. The graph is the quantitative data from the transmission electron microscopy. Data are presented as means ± SEM *P<0.05 compared with NC group; ^#P<0.05 compared to Ang II + NC.