<i>Ex vivo</i> organs of infected mice confirming the systemic dissemination of the parasite and the brain commitment in late phases of infection.

<p>Detection of bioluminescence <i>ex-vivo</i> in organs isolated from mice injected intraperitoneally with 10²<i>Tv</i>LrDNA-luc BSF. Organs were rinsed in PBS and D-Luciferin was added before light detection. Organs from a representative mouse out of 3 for each time point are depicted. Color scale to the right of the pictures encodes for signal intensity (photons/second). The values of each animal have been taken into consideration to obtain the arithmetic means of bioluminescence ×10⁵ ph./s ± the Standard Deviations of the means and are indicated underneath the pictures. (<b>A</b>) spleen, (<b>B</b>) liver, (<b>C</b>) lungs and heart (white arrows) and (<b>D</b>) brain. Even if individual variations between the organs pertaining to the same group of mice are observed for each time point, they do not influence the data interpretation.