<i>Ex vivo</i> bioluminescence imaging of the internal organs of chronically-infected mice.

<p>Gastro-intestinal (GI) tract, heart (H), skeletal muscle (SkM), liver (Li), lung (Lu), spleen (Sp) and mesenteric fat (F) are shown uniformly positioned on a petri dish surface as indicated in the top left image. Each group of mice is represented by one image labeled with the animal gender, compound name and a number of <i>T</i>. <i>cruzi</i>-positive animals in the group of five. Collectively, bioluminescence was detected in the GI of all <i>T</i>. <i>cruzi</i>-positive animals and randomly in heart, skeletal muscle, liver and mesenteric fat. Animals in the <b>9</b>- or <b>12-</b>treated groups showed <i>T</i>. <i>cruzi</i> bioluminescence in the GI tract, liver, lung and mesenteric fat. Bioluminescence was not detected in one female treated with <b>9</b> and two females treated with <b>12</b>. Quantitative analysis of all animals is shown in <a href="http://www.plosntds.org/article/info:doi/10.1371/journal.pntd.0006132#pntd.0006132.g008" target="_blank">Fig 8</a>.